Advantages of using a Vacuum Cleaner as blower There are some advantages in using vacuum cleaners, which may not be provided in a Laminar Flow Hood I have provided the filter dimensions of 0.29 meters width by 0.15 meters height, considering the size of HEPA filter I could lay my hands on. With 0.020 cubic meter of air per second, let us see how much work area it can cover Air flow provided by the blower = width of HEPA filter x height of HEPA filter x air speed required therefore, Work area (width x height) = air flow / air speed = 0.020 / 0.50 = 0.04 square meter Now, if we provide HEPA filter area of 0.27 meter width x 0.15 meter height, the available work area will be 0.04 square meters, which is sufficient for the effective functioning of the flow hood. I have removed the outer most filter from the outlet for connecting the outlet pipe, and used the HEPA filter instead. The blower efficiency of the model I have used is 20 liters per second, that is 0.020 cubic meters per second including all filters. Work area provided by the blower Laminar flow hoods are designed to provide an air-flow of about 0.5 meters per second in the working area. I have removed the outer-most filter and provided a cheaper version of High Efficiency Particulate Air (HEPA) filter between the air chamber and the work area. I have also added a pre-filter to remove any heavier particles before the air is sucked into the vacuum cleaner. The next three stages involve the air to pass through specially designed built-in electric filters with carbon which blows out completely pure fresh air. This bag can be removed, cleaned and then placed again in position. This air then passes through a cloth bag, which filters out any particle which escapes through the paper bag. However, for better results, we can use fresh bag whenever we use the laminar flow hood. This paper bag is replaceable and can be used for up to five times. The first stage involves the incoming air to pass through a special particle, anti-microbial, biocide treated paper filter which has been treated for protection against bacteria, fungus and yeast. Filtration: The model I used consists of unique five stage filtration process and the air coming out after filtration is pure and fresh. Let us check out the facts for its suitability for quality and effectiveness for being used in a flow hood. This Instructable will provide you step-by-step instructions on how to build your own portable Laminar Flow Hood without any compromise on standard specifications.Īre Vacuum Cleaners Suitable for Laminar Flow Hoods as blowers.? I have used Euroclean Star model vacuum cleaner to be used as blower in the flow hood. In a vertical type, the air is blown from the top over the working area and moves out through the base I have carried out extensive study and came out with a design for constructing a Horizontal type Laminar Flow Hood, making use of a vacuum cleaner for the blower and press board / Plexiglas for the hood body thus reducing the cost considerably. In a horizontal type, the purified air is blown from the back and moves towards the front of the working area. There are two main types of Laminar Flow Hoods : Horizontal Airflow and Vertical Airflow. The alternative.? Construct your own equipment. large corporations, universities, and research institutions can afford this but individual hobbyists like me can not. the one with the very lowest specifications costs more than one thousand US Dollars. However, the cost of the simplest form of the equipment is very prohibitive. A Laminar Flow Hood provides a small sterile work area, where you can carry out all your culture works without the risk of contamination, simply by flowing completely purified air at a specified rate. One equipment I wanted most but beyond my reach was a Laminar Flow Hood. My interest in Mycology and Tissue culture started long back which led me to collect few laboratory equipment like Petri dishes, test tubes, weighing equipment, measuring glasses and also chemicals like Hydrogen Peroxide, Agar-agar and Potassium Permanganate.
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